miRNA profiling from blood — challenges and recommendations
نویسندگان
چکیده
The discovery of stable miRNA species circulating in blood has led to increased research focus on disease-related variations in serum and plasma miRNA expression and the possibility that such variations could serve as noninvasive biomarkers for disease. Working with serum and plasma miRNA presents various challenges in purification and characterization. In this paper, we outline QIAGEN® recommendations for robust purification and quantification, as well as reliable data normalization and analysis. Jonathan Shaffer,1 Martin Schlumpberger,2 and Eric Lader1 1 QIAGEN Inc., 6951 Executive Way, Frederick, MD, USA; 2 QIAGEN GmbH, QIAGEN Strasse 1, Hilden, Germany. miRNA profiling from blood — challenges and recommendations stabilization, and purification solutions, as well as a robust miRNA quantification system that enables efficient profiling of miRNA changes in serum, plasma, and many other sample types.
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